Analysis Note

ControlIncluded free of charge with the antibody is 40 µg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 µL of sterile deionized water. For negative control, preincubate 1 µg of peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Application

Western blot: 1:200 using ECL on rat cerebellum lysate and HEK- K_2P4.1 transfected cells.
Immunohistochemistry on rat brain sections.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.

Research Sub CategoryIon Channels & Transporters

Research CategoryNeuroscience

Anti-K2P4.1 Antibody is an antibody against K2P4.1 for use in IH & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Purified peptide from amino acids 343-359 of human K_2P4.1

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.025% sodium azide as a preservative. Reconstitute with 50 µL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

ImmunoAffinity Purified

Specificity

The immunogen sequence is identical in mouse, rat and canine.

Recognizes K_2P4.1 (TRAAK, TWIK-related arachidonic acid-stimulated K+ channel, KCNK4).

Storage and Stability

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.